The ABCC8 Polyclonal Antibody (CAB8456) is a vital tool for researchers studying the ABCC8 gene, which encodes the sulfonylurea receptor 1 (SUR1) subunit of the pancreatic beta-cell ATP-sensitive potassium (KATP) channel. This antibody is produced in rabbits and demonstrates high reactivity with human samples, making it ideal for use in Western blot applications.The ABCC8 gene is essential for glucose-stimulated insulin secretion in pancreatic beta cells, making it a crucial target for research into diabetes and other metabolic disorders. The ABCC8 Polyclonal Antibody enables the detection and analysis of ABCC8 protein expression in various cell types, offering insights into the regulation of insulin secretion and the pathogenesis of diabetes.
With its ability to specifically bind to ABCC8, this antibody is a valuable tool for studies in molecular biology, endocrinology, and pharmacology. Researchers can use it to investigate the role of ABCC8 in insulin secretion, glucose metabolism, and the development of diabetes, paving the way for the development of new therapeutic strategies for managing metabolic diseases.
Product Name:
ABCC8 Rabbit Polyclonal Antibody
SKU:
CAB8456
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 700-1000 of human ABCC8 (NP_000343.2).
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations in the ABCC8 gene and deficiencies in the encoded protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6833
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse pancreas, using ABCC8 antibody (CAB8456) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
