The ABCC2 Polyclonal Antibody (CAB8405) is specifically designed for research involving the ABCC2 protein, also known as the multidrug resistance-associated protein 2 (MRP2). This cell membrane transporter is involved in the efflux of conjugated organic anions and drugs, playing a crucial role in drug metabolism and detoxification processes.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the ABCC2 protein, enabling accurate detection and analysis in various cell types. This makes it an ideal tool for studies in pharmacology, toxicology, and drug resistance research.
The ABCC2 protein is known to be overexpressed in certain cancers, leading to multidrug resistance and impacting the effectiveness of chemotherapy treatments. Understanding the role of ABCC2 in drug efflux mechanisms is essential for the development of strategies to overcome resistance and improve the efficacy of cancer therapies.Overall, the ABCC2 Polyclonal Antibody (CAB8405) is a valuable tool for researchers aiming to unravel the intricate mechanisms of drug transport and resistance, paving the way for advancements in personalized medicine and targeted cancer therapy.
Product Name:
ABCC2 Rabbit Polyclonal Antibody
SKU:
CAB8405
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-315 of human MRP2/ABCC2 (NP_000383.1).
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
Purification Method:
Affinity purification
Gene ID:
1244
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (CAB8405) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
