The ABCB7 Polyclonal Antibody (CAB14699) is specifically designed for research involving ABCB7, a protein that plays a crucial role in cellular iron metabolism and mitochondrial function. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications such as Western blot and immunofluorescence. It binds specifically to the ABCB7 protein, allowing for precise detection and analysis in a variety of cell types.ABCB7, also known as ATP-binding cassette sub-family B member 7, is essential for the transport of iron/sulfur clusters into the mitochondria, where they are necessary for the function of various mitochondrial enzymes.
Disruption of ABCB7 function has been linked to disorders such as X-linked sideroblastic anemia with ataxia, making it a key target for research in the field of iron metabolism and mitochondrial biology.Furthermore, understanding the role of ABCB7 in cellular iron metabolism is crucial for developing potential therapies for disorders related to iron overload or deficiency. The ABCB7 Polyclonal Antibody (CAB14699) offers researchers a valuable tool for studying the function and regulation of ABCB7, ultimately advancing our knowledge of iron homeostasis and mitochondrial function in health and disease.
Product Name:
ABCB7 Rabbit Polyclonal Antibody
SKU:
CAB14699
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 503-753 of human ABCB7 (NP_004290.2).
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a half-transporter involved in the transport of heme from the mitochondria to the cytosol. With iron/sulfur cluster precursors as its substrates, this protein may play a role in metal homeostasis. Mutations in this gene have been associated with mitochondrial iron accumulation and isodicentric (X)(q13) and sideroblastic anemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
22
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ABCB7 Rabbit pAb (CAB14699) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
