P Glycoprotein Rabbit Polyclonal Antibody (CAB14765)
The ABCB1 Polyclonal Antibody (CAB14765) is a powerful tool for researchers studying the ABCB1 protein, also known as P-glycoprotein, which plays a crucial role in multi-drug resistance in cancer cells. This antibody, produced in rabbits, is highly specific for human ABCB1 samples and has been validated for use in Western blotting applications.ABCB1 is an important transporter protein located on the cell membrane that actively pumps a variety of chemotherapeutic agents out of cancer cells, leading to treatment resistance.
By targeting and detecting the ABCB1 protein, researchers can gain insight into the mechanisms of drug resistance and potentially develop strategies to overcome it in cancer therapy.Studies on ABCB1 have significant implications for cancer research and the development of more effective treatment strategies. By utilizing the ABCB1 Polyclonal Antibody, researchers can further explore the role of this protein in drug resistance and potentially identify new therapeutic targets for improving cancer treatment outcomes.
Product Name:
P Glycoprotein Rabbit Polyclonal Antibody
SKU:
CAB14765
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 590-689 of human P Glycoprotein (NP_000918.2).
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; p-170; P Glycoprotein
Positive Sample:
Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
141kDa
Observed MW:
160kDa
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using P Glycoprotein Rabbit pAb (CAB14765) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
