P Glycoprotein Rabbit Polyclonal Antibody (CAB1284)
The ABCB1 Polyclonal Antibody (CAB1284) is a specialized tool for researchers studying the ABCB1 gene, which encodes a membrane transporter protein involved in drug resistance and cellular detoxification mechanisms. This antibody, produced in rabbits, exhibits high specificity for human samples and has been rigorously validated for use in Western blot applications. By targeting the ABCB1 protein, this antibody enables precise detection and analysis of drug transport mechanisms in a variety of cell types, making it an indispensable tool for studies in pharmacology, drug development, and cancer research.
ABCB1, also known as P-glycoprotein, plays a crucial role in multidrug resistance in cancer cells by actively pumping chemotherapeutic agents out of the cell, thereby reducing their effectiveness. Understanding the functionality and regulation of ABCB1 is essential for developing strategies to overcome drug resistance in cancer treatment and improve the efficacy of chemotherapy. With its high specificity and reliability, the ABCB1 Polyclonal Antibody (CAB1284) is a valuable asset for researchers aiming to uncover the mechanisms underlying drug resistance and identify novel therapeutic targets.
Product Name:
P Glycoprotein Rabbit Polyclonal Antibody
SKU:
CAB1284
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 630-710 of human P Glycoprotein (NP_000918.2).
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; p-170; P Glycoprotein
Positive Sample:
Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
141kDa
Observed MW:
160kDa
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using P Glycoprotein antibody (CAB1284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
