P Glycoprotein Rabbit Polyclonal Antibody (CAB11747)
The ABCB1 Polyclonal Antibody (CAB11747) is a valuable tool for research involving the ABCB1 gene, which encodes a protein involved in drug resistance and transport. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the ABCB1 protein, allowing for its detection and analysis in various cell types.ABCB1, also known as P-glycoprotein, plays a crucial role in the efflux of drugs and toxins out of cells, contributing to multidrug resistance in cancer cells.
Understanding the function and regulation of ABCB1 is essential for developing strategies to overcome drug resistance in cancer treatment. This antibody is therefore ideal for studies in pharmacology, oncology, and drug discovery, providing valuable insights into the mechanisms of drug resistance and potential therapeutic targets.
Product Name:
P Glycoprotein Rabbit Polyclonal Antibody
SKU:
CAB11747
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 603-692 of human P Glycoprotein (NP_000918.2).
Sequence:
DGVI VEKG NHDE LMKE KGIY FKLV TMQT AGNE VELE NAAD ESKS EIDA LEMS SNDS RSSL IRKR STRR SVRG SQAQ DRKL STKE ALDE SI
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; p-170; P Glycoprotein
Positive Sample:
Mouse liver,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
141kDa
Observed MW:
160-200kDa
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using P Glycoprotein Rabbit pAb (CAB11747) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
