The ABCA1 Polyclonal Antibody (CAB16337) is a valuable tool for researchers studying the ABCA1 protein, a key player in cellular cholesterol homeostasis and lipid transport. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.ABCA1 is a transmembrane protein that plays a crucial role in the efflux of cholesterol and phospholipids from cells, playing a key role in the formation of high-density lipoprotein (HDL) particles. Dysregulation of ABCA1 has been linked to various lipid metabolism disorders, cardiovascular diseases, and neurodegenerative conditions.
By using the ABCA1 Polyclonal Antibody, researchers can accurately detect and analyze ABCA1 expression in a variety of cell types, providing valuable insights into the mechanisms underlying cholesterol metabolism. This antibody is an essential tool for studies in lipid biology, atherosclerosis, and metabolic disorders, ultimately contributing to the development of novel therapeutic approaches targeting ABCA1.
Product Name:
ABCA1 Rabbit Polyclonal Antibody
SKU:
CAB16337
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1170-1350 of human ABCA1 (NP_005493.2).
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency.
Purification Method:
Affinity purification
Gene ID:
19
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Mouse heart, using ABCA1 Rabbit pAb (CAB16337) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
