The ABAT Rabbit Monoclonal Antibody (CAB9146) is a high-quality antibody developed for research involving ABAT, an enzyme involved in the catabolism of gamma-aminobutyric acid (GABA) in the brain. This antibody, derived from rabbit monoclonal cells, is specifically designed for use in various applications such as Western blot and immunohistochemistry.ABAT plays a crucial role in GABA metabolism, a neurotransmitter that regulates neuronal excitability in the brain. Dysregulation of ABAT activity has been implicated in neurological disorders such as epilepsy, autism, and schizophrenia.
This antibody binds specifically to ABAT, allowing for the accurate detection and analysis of ABAT protein levels in tissue samples.Research into ABAT function and regulation is essential for understanding the mechanisms underlying neurological disorders and developing potential therapeutic interventions. The ABAT Rabbit Monoclonal Antibody (CAB9146) provides researchers with a valuable tool for studying ABAT expression and activity in various biological contexts, paving the way for advancements in neuroscience and drug development.
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
18
Clone Number:
ARC1450
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using GABA transaminase (GABA transaminase (ABAT)) Rabbit mAb (CAB9146) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
