The ABAT Polyclonal Antibody (CAB5299) is a specialized tool for researchers studying ABAT, an enzyme involved in neurotransmitter metabolism and GABAergic signaling. This antibody, produced through rabbit immunization, has a high affinity for human samples and has been tested for efficacy in Western blot applications. By binding specifically to the ABAT protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations related to neuroscience and neurological disorders.ABAT is a key enzyme in the catabolism of GABA, an important neurotransmitter in the central nervous system.
Dysregulation of ABAT activity has been implicated in various neurological conditions, including epilepsy and neurodevelopmental disorders. By studying the role of ABAT in neurotransmission, researchers can gain insights into the pathophysiology of these disorders and potentially identify new therapeutic targets for intervention. The ABAT Polyclonal Antibody is a valuable tool for advancing our understanding of GABAergic signaling and its implications for neurological health.
Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human GABA transaminase (GABA transaminase (ABAT)) (NP_001120920.1).
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
18
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GABA transaminase (ABAT) Rabbit pAb (CAB5299) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
