The AAK1 Polyclonal Antibody is a powerful tool for researchers studying the AAK1 protein, a key regulator of cellular processes involved in cancer and other diseases. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By targeting the AAK1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in oncology and drug development. AAK1, also known as AP2 associated protein kinase 1, plays a crucial role in controlling cell growth, proliferation, and survival, making it a promising target for therapeutic intervention.
Research on AAK1 can provide insights into potential treatment strategies for cancer and other diseases where dysregulation of cellular processes is involved. By understanding the function of AAK1, researchers can advance the development of targeted therapies that aim to modulate cell signaling pathways for improved clinical outcomes.
Product Name:
AAK1 Polyclonal Antibody
SKU:
CAB22105
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human AAK1 (NP_055726.4).
This gene encodes a member of the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The encoded protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. This kinase has been shown to play an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of this kinase are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Purification Method:
Affinity purification
Gene ID:
22848
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using AAK1 Rabbit pAb (CAB22105) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
