The AAK1 Polyclonal Antibody (PAC021868) is a valuable tool for research into AAK1, a protein kinase involved in various cellular processes including endocytosis and cell proliferation. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AAK1 protein, it allows for the detection and analysis of AAK1 expression in different cell types, making it an ideal choice for studies in cell biology and cancer research.AAK1, known for its role in clathrin-mediated endocytosis, is implicated in the regulation of various signaling pathways and cellular functions.
Its involvement in processes such as receptor internalization and intracellular trafficking highlights its importance in maintaining cell homeostasis. Research into the function of AAK1 is essential for understanding its potential as a therapeutic target in diseases related to aberrant cell signaling and trafficking, including cancer and neurodegenerative disorders. The AAK1 Polyclonal Antibody provides researchers with a reliable tool for investigating the role of AAK1 in these conditions and advancing our knowledge of cellular biology.
Antibody Name:
AAK1 Antibody (PACO21868)
Antibody SKU:
PACO21868
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human AAK1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from COS7 cells, using AAK1 antibody.
AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.
UniProt Protein Details:
Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NAK family
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button
Molecular Function:Notch binding; protein binding; protein serine/threonine kinase activity
Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization
NCBI Summary:
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
