The AAC5 Antibody (PAC053954) is a polyclonal antibody designed for research involving AAC5, a key protein involved in various cellular processes. This antibody, generated in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the AAC5 protein, this antibody enables the detection and analysis of AAC5 expression in a variety of cell types, making it an excellent tool for studies in molecular biology and cell physiology.
AAC5, also known as a critical regulator of cellular function, plays a vital role in processes such as metabolism, energy production, and cell growth. Its importance in these pathways makes it a valuable target for research into diseases like neurodegenerative disorders, metabolic syndrome, and cancer. Understanding the functions of AAC5 is essential for developing therapies that target or modulate its activity in the context of these diseases.
Western Blot. Positive WB detected in: HEK293 whole cell lysate. All lanes: AACS antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 76, 68, 31 kDa. Observed band size: 76 kDa.
Immunoprecipitating AACS in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO53954 in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO53954 (8µg) + HEK293 whole cell lysate (500µg). Lane 3: HEK293 whole cell lysate (10µg).
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO53954 at dilution of 1:100.
Background:
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid, synthesis during adipose tissue development (By similarity).
Synonyms:
Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Acyl-CoA synthetase family member 1) (Protein sur-5 homolog), AACS, ACSF1
UniProt Protein Function:
AACS: Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. Belongs to the ATP-dependent AMP-binding enzyme family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.2.1.16; Carbohydrate Metabolism - butanoateChromosomal Location of Human Ortholog: 12q24.31Cellular Component: cytosolMolecular Function: acetoacetate-CoA ligase activity; protein bindingBiological Process: ketone body biosynthetic process
