The Arachidonic Acid ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of arachidonic acid levels in biological samples. Arachidonic acid is an important fatty acid involved in various physiological processes, including inflammation, blood clotting, and cell signaling.This kit provides researchers with a reliable and reproducible method for measuring arachidonic acid levels in serum, plasma, and cell culture supernatants. By accurately determining arachidonic acid levels, researchers can gain valuable insights into the role of this fatty acid in various diseases and biological processes.
Whether studying inflammation, cardiovascular diseases, or neurological disorders, the Arachidonic Acid ELISA Kit is a valuable tool for researchers looking to further their understanding of arachidonic acid and its impact on human health. Get accurate and reliable results with this easy-to-use kit for a wide range of research applications.
Product Name:
AA (Arachidonic Acid) ELISA Kit (UNES00032)
SKU:
UNES00032
Target:
AA (Arachidonic Acid) (UNES00032)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with AA. During the reaction, AA in the sample or standard competes with a fixed amount of AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to AA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
100-111
93-108
Average (%)
105
105
99
1:4
Range (%)
88-101
90-103
95-111
Average (%)
93
97
102
1:8
Range (%)
87-98
91-103
94-111
Average (%)
92
97
102
1:16
Range (%)
84-97
87-98
100-113
Average (%)
90
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
90-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
9.1
36.7
4.7
8.7
36.6
Standard deviation
0.3
0.4
1.8
0.2
0.4
1.9
C V (%)
6.38
4.4
4.9
4.26
4.6
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of AA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes AA in samples. No significant cross-reactivity or interference between AA and analogues was observed.
