The A1CF Polyclonal Antibody (CAB13087) is a crucial tool for researchers studying A1CF, a protein known for its role in RNA processing and regulation. This antibody, generated in rabbits, shows high specificity and reactivity towards human samples, making it an excellent choice for Western blot applications. By binding to the A1CF protein, researchers can accurately detect and analyze its expression in various cell types, providing valuable insights for studies in molecular biology, gene expression, and RNA processing mechanisms.
A1CF, also known as apobec1 complementation factor, is essential for the editing and processing of specific RNA transcripts, ultimately influencing gene expression and protein production. Its involvement in these critical cellular processes highlights its importance in various biological pathways and diseases. Research on A1CF is particularly relevant in areas such as cancer biology, gene regulation, and RNA editing mechanisms, where understanding its function can lead to the development of targeted therapies and interventions.
Product Name:
A1CF Rabbit Polyclonal Antibody
SKU:
CAB13087
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human A1CF (NP_055391.2).
Sequence:
APPE RGCE IFIG KLPR DLFE DELI PLCE KIGK IYEM RMMM DFNG NNRG YAFV TFSN KVEA KNAI KQLN NYE
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
ACF; ASP; ACF64; ACF65; APOBEC1CF; A1CF
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Endoplasmic reticulum, Nucleus.
Calculated MW:
65kDa
Observed MW:
65kDa
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
29974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using A1CF Rabbit pAb (CAB13087) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
