The 8-OHdG (8-hydroxydeoxyguanosine) ELISA Kit is specifically designed for the accurate quantification of 8-OHdG levels in biological samples such as urine, plasma, serum, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.8-OHdG is a well-known marker of oxidative DNA damage and is commonly used in studying oxidative stress-related diseases such as cancer, cardiovascular diseases, and aging. By accurately measuring 8-OHdG levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially develop novel therapeutic strategies. Overall, the 8-OHdG ELISA Kit provides researchers with a powerful tool for investigating oxidative stress, biomarker discovery, and drug development in a reliable and efficient manner.
Product Name:
8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
SKU:
UNES00018
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
92-104
87-101
90-103
Average (%)
98
92
95
1:4
Range (%)
89-106
82-94
83-95
Average (%)
97
88
90
1:8
Range (%)
93-106
85-100
88-99
Average (%)
100
92
94
1:16
Range (%)
87-102
86-99
88-101
Average (%)
94
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
91-104
98
Cell Culture Media (n=8)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.83
10.34
45.93
5.17
9.54
47.36
Standard deviation
0.3
0.72
2.91
0.46
0.82
2.99
C V (%)
6.11
6.98
6.34
8.82
8.61
6.31
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 50 μL
Specificity:
This kit recognizes Universal 8-OHdG in samples.No significant cross-reactivity or interference between Universal 8-OHdG and analogues was observed
Storage:
2-8℃
Research Area:
Metabolism,Cardiovascular,Immunology,Cancer
Rus, A, L., et al.
Serum 8-OHdG as a Marker of Oxidative DNA Damage in Acute Exacerbations of COPD: Associations with GOLD Stage and Smoking Status—A Cross Sectional Study
