The 5-Oxoprolinase Polyclonal Antibody (CAB12032) is a valuable tool for researchers studying the 5-oxoprolinase enzyme, which is involved in the glutathione metabolism pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.5-Oxoprolinase is a key enzyme in the gamma-glutamyl cycle, playing a crucial role in maintaining cellular redox homeostasis and protecting cells from oxidative damage. Dysregulation of this enzyme has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes.
By specifically targeting the 5-oxoprolinase enzyme, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential tool for researchers in biochemistry, molecular biology, and pharmaceutical development. Understanding the role of 5-oxoprolinase in cellular metabolism and disease pathogenesis is crucial for the development of targeted therapies and diagnostic tools in the future.
Product Name:
OPLAH Rabbit Polyclonal Antibody
SKU:
CAB12032
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1).
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Purification Method:
Affinity purification
Gene ID:
26873
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using OPLAH Rabbit pAb (CAB12032) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
