The 5'-Nucleotidase Polyclonal Antibody (CAB2029) is a vital tool for researchers studying 5'-Nucleotidase, an enzyme involved in the metabolism of nucleotides and implicated in various physiological and pathological processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the 5'-Nucleotidase protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an indispensable tool for investigations in fields such as biochemistry, cell biology, and drug development.
5'-Nucleotidase plays a crucial role in nucleotide metabolism and purine salvage pathways, making it a key player in cellular processes like DNA synthesis, energy metabolism, and signal transduction. Dysregulation of this enzyme has been linked to diseases such as cancer, metabolic disorders, and autoimmune conditions, underscoring the importance of studying its function and regulation. By using the 5'-Nucleotidase Polyclonal Antibody, researchers can gain valuable insights into the role of this enzyme in health and disease, potentially leading to the development of novel therapeutic strategies.
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4907
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit pAb (CAB2029) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
