4-HNE (4-Hydroxynonenal) ELISA Kit (AEES00007)
- SKU:
- AEES00007
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Size:
- 96 Assays
- Sensitivity:
- 0.38 ng/mL
- Range:
- 0.63-40 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | 4-HNE (4-Hydroxynonenal) ELISA Kit |
SKU: | AEES00007 |
Target: | 4-HNE (4-Hydroxynonenal) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 0.38 ng/mL |
Detection range: | 0.63-40 ng/mL |
Kit component: |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed. |