The 25-Hydroxy Vitamin D₃ ELISA Kit from Assay Genie is a reliable tool for the quantitative detection of 25-hydroxy vitamin D₃ levels in serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.25-hydroxy vitamin D₃ is a key indicator of vitamin D status in the body, playing a crucial role in regulating calcium and phosphate levels, as well as supporting overall bone health and immune function. Deficiencies in 25-hydroxy vitamin D₃ have been linked to a range of health conditions, making it a valuable biomarker for studying and monitoring vitamin D deficiency-related diseases.
With the 25-Hydroxy Vitamin D₃ ELISA Kit, researchers can efficiently measure 25-hydroxy vitamin D₃ levels in samples, enabling them to better understand the impact of vitamin D on health and potentially develop targeted interventions for improving vitamin D status. Trust Assay Genie for accurate and reliable results in your research endeavors.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 25-HVD3. During the reaction, 25-HVD3 in the sample or standard competes with a fixed amount of 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
97-112
97-111
Average (%)
98
102
103
1:4
Range (%)
91-101
99-113
99-112
Average (%)
96
105
105
1:8
Range (%)
90-103
93-110
97-111
Average (%)
96
100
105
1:16
Range (%)
92-104
97-111
92-108
Average (%)
98
103
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.44
28.15
88.19
9.08
27.46
85.89
Standard deviation
0.62
1.47
2.88
0.52
1.21
4.08
C V (%)
6.57
5.22
3.27
5.73
4.41
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 25-HVD3 in samples. No significant cross-reactivity or interference between 25-HVD3 and analogues was observed.
