2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody (PACO60519)
- SKU:
- PACO60519
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- ICC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody (PACO60519)
The 2-Hydroxyisobutyryl-HIST1H2BC-K12 Antibody (PACO60519) is a valuable tool for researchers studying protein modifications related to histones, the proteins responsible for packaging DNA in the cell nucleus. This polyclonal antibody, generated in rabbits, specifically targets the 2-hydroxyisobutyryl modification on the histone variant HIST1H2BC-K12. Histone modifications play a critical role in regulating gene expression, DNA replication, and repair processes. The detection and analysis of specific histone modifications, such as 2-hydroxyisobutyrylation, can provide insight into cellular processes and disease mechanisms. This antibody is validated for use in various applications, including Western blotting, immunofluorescence, and chromatin immunoprecipitation, making it a versatile tool for researchers in the fields of epigenetics, cancer biology, and chromatin remodeling.
By targeting the 2-hydroxyisobutyryl modification on histone proteins, this antibody offers researchers the ability to investigate the role of this specific modification in chromatin structure and function. Understanding the impact of histone modifications on gene regulation and cellular processes is essential for developing targeted therapies for diseases associated with epigenetic dysregulation. The 2-Hydroxyisobutyryl-HIST1H2BC-K12 Antibody (PACO60519) provides a valuable resource for
| Antibody Name: | 2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody (PACO60519) |
| Antibody SKU: | PACO60519 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, ICC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, ICC:1:10-1:100, IF:1:1-1:10 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence around site of 2-hydroxyisobutyryl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h. All lanes: HIST1H2BC antibody at 1:500. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa. |
 | Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with PACO60519 at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunocytochemistry analysis of PACO60519 diluted at 1:15 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK |
| UniProt Protein Function: | H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P62807 |
| NCBI GenInfo Identifier: | 290457686 |
| NCBI Gene ID: | 8339 |
| NCBI Accession: | P62807.4 |
| UniProt Secondary Accession: | P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080, |
| UniProt Related Accession: | P62807 |
| Molecular Weight: | 13,906 Da |
| NCBI Full Name: | Histone H2B type 1-C/E/F/G/I |
| NCBI Synonym Full Names: | histone cluster 1, H2bg |
| NCBI Official Symbol: | HIST1H2BGÂ Â |
| NCBI Official Synonym Symbols: | H2B/a; H2BFA; H2B.1A; dJ221C16.8Â Â |
| NCBI Protein Information: | histone H2B type 1-C/E/F/G/I |
| UniProt Protein Name: | Histone H2B type 1-C/E/F/G/I |
| UniProt Synonym Protein Names: | Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H2BCÂ Â |
| UniProt Entry Name: | H2B1C_HUMAN |
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