The 14-3-3 Sigma Polyclonal Antibody is a valuable tool for researchers studying the 14-3-3 Sigma protein, a key player in cellular signaling and regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications, allowing for easy detection and analysis of the 14-3-3 Sigma protein in various cell types.The 14-3-3 Sigma protein is involved in diverse cellular processes, including cell cycle regulation, apoptosis, and cell adhesion.
Its dysregulation has been associated with various diseases, making it a promising target for research in cancer, neurodegenerative disorders, and other pathologies. By using the 14-3-3 Sigma Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of this protein, advancing our understanding of its role in health and disease.
Product Name:
14-3-3 sigma Polyclonal Antibody
SKU:
CAB21438
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human 14-3-3 sigma (NP_006133.1).
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
Purification Method:
Affinity purification
Gene ID:
2810
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from wild type(WT) and 14-3-3 sigma knockout (KO) HeLa(KO) cells, using 14-3-3 sigma antibody (CAB21438) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
