14-3-3 gamma Polyclonal Antibody (CAB21651)
- SKU:
- CAB21651
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | 14-3-3 gamma Polyclonal Antibody |
| SKU: | CAB21651 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human 14-3-3 gamma (P61981). |
| Sequence: | VLSL LDNY LIKN CSET QYES KVFY LKMK GDYY RYLA EVAT GEKR ATVV ESSE KAYS EAHE ISKE HMQP THPI RLGL ALNY SVFY YEIQ NAPE QACH LAKT A |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 |
| Synonyms: | DEE56; EIEE56; PPP1R170; 14-3-3GAMMA; 14-3-3 gamma |
| Positive Sample: | U-251 MG,K-562,HeLa,Mouse brain,Mouse heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm. |
| Calculated MW: | 28kDa |
| Observed MW: | Refer to figures |
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
| Purification Method: | Affinity purification |
| Gene ID: | 7532 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using 14-3-3 gamma Rabbit pAb (CAB21651) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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