The 14-3-3 Epsilon Rabbit Monoclonal Antibody (CAB4933) is a valuable tool for researchers studying the 14-3-3 Epsilon protein, a member of the 14-3-3 family that plays a critical role in cell signaling and regulation. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the 14-3-3 Epsilon protein, this antibody enables the detection and analysis of this important regulatory molecule in various cell types.The 14-3-3 Epsilon protein is involved in diverse cellular processes, including cell cycle regulation, apoptosis, and signaling pathways. Dysregulation of 14-3-3 Epsilon has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases.
By studying the function and expression of 14-3-3 Epsilon, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the 14-3-3 Epsilon Rabbit Monoclonal Antibody (CAB4933) is a reliable tool for researchers investigating the role of 14-3-3 Epsilon in health and disease. Its specificity, sensitivity, and compatibility with various experimental techniques make it a valuable asset for studies in cell biology, biochemistry, and molecular biology.
Product Name:
14-3-3 epsilon Rabbit Monoclonal Antibody
SKU:
CAB4933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 epsilon (P62258).
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7531
Clone Number:
ARC1222
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon Rabbit mAb (CAB4933) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
