The 14-3-3 Alpha/Beta Rabbit Monoclonal Antibody (CAB9151) is a valuable tool for researchers studying the 14-3-3 family of proteins, which play important roles in various cellular processes such as signal transduction, cell cycle regulation, and apoptosis. This antibody, raised in rabbits, is highly specific for detecting both 14-3-3 alpha and beta isoforms in human samples, making it an essential tool for Western blot and immunohistochemistry applications.The 14-3-3 proteins are known to interact with a wide range of target proteins, influencing their localization, stability, and activity.
By targeting the alpha and beta isoforms specifically, this antibody enables researchers to study the role of 14-3-3 in different cellular pathways and processes. Its high reactivity and specificity make it an ideal choice for studies in cell biology, cancer research, and neurodegenerative diseases where 14-3-3 proteins have been implicated.Overall, the 14-3-3 Alpha/Beta Rabbit Monoclonal Antibody (CAB9151) is a reliable tool for investigating the functions of 14-3-3 alpha and beta isoforms in various biological contexts, providing valuable insights into their roles in health and disease.
Product Name:
14-3-3 alpha/beta Rabbit Monoclonal Antibody
SKU:
CAB9151
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 147-246 of human 14-3-3 alpha/beta (P31946).
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
7529
Clone Number:
ARC1453
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using 14-3-3 alpha/beta Rabbit mAb (CAB9151) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
